Fig. 2. Identified peptide has a specific affinity to BACE1 when incubated with homologous BACE2, cathepsin D and renin. (a) Immobilised BACE1, BACE2, cathepsin D and renin were incubated with a 100nM solution containing the peptide isolated from the phage display and biopanning experiments. (b) Testing overall peptide affinity for BACE1, individual homologs were preincubated with the peptide then incubated to an immobilized BACE1. A combined solution containing all homologs represents the total non-specific binding potential with these specific proteins. The binding occurring between the immobilized target and peptide is represented by an increase in colorimetric response. All values are reported as individual data points (n=3) and the bars represent mean binding. Statistical significance is determined by unpaired Student's t-test (p<0.001 ***, p<0.0001 ****).